TLR9/NFκB-Luc/HEK293
CBP74091
| I. Background | |
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Toll 樣受體（TLR）是一種模式識別受體（PRR），高度保守，可識別各種類型的微 生物病原體相關模式分子（PAMP）。在人類中發(fā)現了 10 個 Toll 樣受體家族（TLR1-10） 成員，在小鼠中發(fā)現了 12 個成員（TLR1-9、TLR11 and TLR13）。TLR1、TLR2、TLR4、 TLR5 和 TLR6 分布在細胞膜表面；TLR3、TLR7、TLR8、TLR9 和 TLR10 分布在細胞內 涵體膜表面（TLR3 識別 dsRNA，TLR9 識別 dsDNA）。TLRs 下游信號的激活主要依賴于 兩類轉錄因子：NF-κB 和干擾調節(jié)因子（IRFs），它們主要誘導促炎細胞因子和 I 型干擾 素（IFN）的產生。 |
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| II. Description | |
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TLR9 NFκB-Luc HEK293 報告基因藥靶模型很好的模擬了體內 TLR9 的信號轉導過程， 原理見下圖所示。
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| II. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | TLR9、NFκB-Luc |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | TLR, TLR-9, tlr, nfkb, nf-kb |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: |
MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1μg/ml puromycin+100μg/ml Hygromycin B
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| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
Figure 2.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of ODN 2006 in TLR9 NFκB-Luc HEK293(C3).
